Hela vimentin

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August undefined, 2024

WebWestern blot analysis of extracts from control HeLa cells (lane 1) or vimentin knockout HeLa cells (lane 2) using Vimentin (5G3F10) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Show More WebJun 22, 2001 · As shown in Figure 4, vimentin in untreated, control HeLa cells (left-hand, upper panel) was organized into an extensive network of cytoplasmic intermediate filaments. However, after 3 h of ...

Regulation of vimentin expression in cultured epithelial cells

WebMay 1, 2009 · The surface vimentin expression pattern observed in HeLa cells is virtually identical to confocal observation of surface vimentin expression previously reported in macrophages . Fixation and staining procedures to verify cell surface and cytosolic staining were verified through the ability to detect β-COP, an intra-Golgi transport marker ... WebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Data were generated using the standard formulation of this product. Show More Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP ® Rabbit mAb. flower abscission

Vimentin (5G3F10) Mouse mAb Cell Signaling Technology

WebSimilar results were obtained using bacterially purified His-tagged Rab7a wt and mutant proteins, in order to pull-down endogenous vimentin from HeLa total extracts (Fig. 2 … WebMar 31, 2024 · At 8 hpi, cells were fixed, nuclei and replication sites were stained with Hoechst and immunostaining was performed to visualise vimentin. In untreated HeLa cells, vimentin was associated with replication sites and newly assembled virions were found throughout the cytoplasm (Figure 3e). As expected, in acrylamide-treated HeLa cells … WebData show that over-expression of vimentin ameliorated cytotoxicity of gambogic acid (GA) in HeLa cells. High vimentin and low beta4 integrin protein levels are associated with poor survival in oral squamous carcinoma patients. PTTG2 induces psoriasis by regulating epidermal expression of vimentin and E-cadherin. flower accounting

Purified Mouse Anti-Vimentin - BD Biosciences

WebWestern blot analysis of extracts from control HeLa cells (lane 1) or vimentin knockout HeLa cells (lane 2) using Vimentin (V9) Mouse mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Show More WebJun 10, 2011 · HeLa cell cytospins spiked in blood from healthy volunteers and processed as per patient samples were used as positive controls for the detection of Twist and vimentin. Positive and negative controls for Twist, vimentin and CK are shown in Figure 1. flower accent recliner coversWebThere are various factors influencing the invasion of AIV into the host cells. In this paper, it was proven that the replication of H9N2 AIV on HeLa and KO-vimentin HeLa cell was … flower abortion

"WebAfter being transfected and screened with puromycin, it was found that compared with the HeLa cell controls, both mRNA level and protein expression of vimentin in the transfected HeLa cells were significantly decreased ( Figure 1 B,C), and vimentin knocked out cells were named KO-vimentin HeLa cells, abbreviated as KO HeLa cells. " - Hela vimentin

Hela vimentin

Vimentin (D21H3) XP® Rabbit mAb (BSA and Azide Free)

WebThe absence of signal in the vimentin knockout HeLa cells confirms specificity of the antibody for vimentin. Western blot analysis of extracts from various cell lines using … WebSep 20, 2013 · For the establishment of the HeLa/endoB2-St and HeLa/vimentin-GFP stable cell lines, cells were transfected as for transient transfections and grown for 48 h in culture medium without G418. After trypsinization and appropriate dilutions, cells were grown for 3 weeks in culture medium supplemented with 0.8 mg/ml G418, and single colonies …

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WebRecognizes endogenous levels of Vimentin protein. Clonality. Monoclonal. Dilution. WB (1/1000 - 1/3000), IHC (1/200 - 1/500), IF/IC (1/100 - 1/200) Gene Symbol. VIM. Storage Condition. Store at -20°C long term. MA9220. 货号规格价格(￥) MA9220M 100μl MA9220S 50μl 以上产品均有现货供应. 订购咨询： orders@ab-mart ... WebThis study represents a comprehensive description of a HeLa xenograft model for in vivo investigations, enabling researchers to assess new treatments for cervical cancer. ...

WebHeLa cell lysate was probed with anti-vimentin (clone RV202, Cat. No. 550513) at concentrations of 120 (lane 1), 60 (lane 2) and 30 ng/ml (lane 3). Vimentin is identified as a band of ~57 kDa. Show More Spectrum Viewer Protocol Library Scientific Resources Catalog No: Your Price: Sign In Unit: Size: 50 µg Request Bulk Reagent Quote Product … WebWestern blot analysis of extracts from HeLa cells, untreated or phosphorylated in vitro by PLK, using Phospho-Vimentin (Ser83) Antibody (upper). β-Actin Antibody #4976 (lower) was used as a loading control. Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP ® Rabbit mAb.

WebMar 2, 2024 · Moreover, in CHIR-treated HeLa cells transiently expressing vimentin BC2T, the nanobody signal was almost exclusively detectable at vimentin fibers (Supplementary Fig. 2c, right panel). WebAll lanes : Anti-Vimentin antibody [V9] - Cytoskeleton Marker at 1 µg/ml Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate Lane 2 : Human tonsil cell lysate Lane 3 : Wild-type HeLa cell lysate Lane 4 : VIM knockout HeLa cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) …

Vimentin was one of the CCAT1 binding proteins and was participated in the tumor metastasis pathway. Knocked down VIM and rescued the downregulation by overexpressing CCAT1 demonstrated that CCAT1 could enhance tumor migration and invasion abilities by stabilizing Vimentin protein.

WebMar 20, 2024 · To further investigate whether vimentin is a functionally relevant target of ajoene’s anti-metastatic activity, we transiently overexpressed vimentin in HeLa and MDA-MB-231 cells (Fig. 6a). A scratch wound was then introduced into the cell monolayer, followed by addition of 10 μM Z-ajoene in DMSO or DMSO alone for 24 h. flower about freedomWebThe absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin. Show More Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using Vimentin (D21H3) XP ® Rabbit mAb #5741. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. flower accessoryWebDec 1, 1992 · Using both vimentin-expressing (HeLa) and vimentin-negative (MCF-7) epithelial cell lines, we have identified the cis-regulatory DNA elements involved in this process. Sequences located 1.1-0.6 kb upstream of the vimentin transcription-initiation site strongly enhance expression in HeLa cells, but are silenced in MCF-7 cells. flower achatWebab154207 staining Vimentin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab154207 at a working dilution of 1 in 100 (shown in pseudo color red) ... greek islands sailing vacationsWebAug 21, 2015 · Vimentin, the major intermediate filament of mesenchymal cells, is mainly involved in tissue integrity and cytoarchitecture 1. The evolutionarily highly conserved protein consists of a central... greek island starting with pWeb(A) The expression of E-cadherin, N-cadherin and Vimentin in transfected HeLa cells was detected by Western blot. (B) The expression of E-cadherin, N-cadherin and Vimentin in transfected SiHa cells was detected by Western blot. Data were presented as mean ± standard deviation with three replicates. flower accountantshttp://www.ab-mart.com.cn/page.aspx?node=%2077%20&id=%2053980 flower abstraction